The lethal effect of a benzamide derivative, 3-methoxybenzamide, can be suppressed by mutations within a cell division gene, ftsZ, in Bacillus subtilis.
نویسندگان
چکیده
3-Methoxybenzamide (3-MBA), which is known to be an inhibitor of ADP-ribosyltransferase, inhibits cell division in Bacillus subtilis, leading to filamentation and eventually lysis of cells. Our genetic analysis of 3-MBA-resistant mutants indicated that the primary target of the drug is the cell division system involving FtsZ function during both vegetative growth and sporulation.
منابع مشابه
Characterization of ftsZ Mutations that Render Bacillus subtilis Resistant to MinC
BACKGROUND Cell division in Bacillus subtilis occurs precisely at midcell. Positional control of cell division is exerted by two mechanisms: nucleoid occlusion, through Noc, which prevents division through nucleoids, and the Min system, where the combined action of the MinC, D and J proteins prevents formation of the FtsZ ring at cell poles or recently completed division sites. METHODOLOGY/PR...
متن کاملBorrelia burgdorferi ftsZ plays a role in cell division.
ftsZ is essential for cell division in many microorganisms. In Escherichia coli and Bacillus subtilis, FtsZ plays a role in ring formation at the leading edge of the cell division septum. An ftsZ homologue is present in the Borrelia burgdorferi genome (ftsZ(Bbu)). Its gene product (FtsZ(Bbu)) is strongly homologous to other bacterial FtsZ proteins, but its function has not been established. Bec...
متن کاملThe structural assembly switch of cell division protein FtsZ probed with fluorescent allosteric inhibitors.
FtsZ is a widely conserved tubulin-like GTPase that directs bacterial cell division and a new target for antibiotic discovery. This protein assembly machine cooperatively polymerizes forming single-stranded filaments, by means of self-switching between inactive and actively associating monomer conformations. The structural switch mechanism was proposed to involve a movement of the C-terminal an...
متن کاملThe effect of MinC on FtsZ polymerization is pH dependent and can be counteracted by ZapA.
The min system prevents polar cell division in bacteria. Here, the biochemical characterization of the interaction of MinC and FtsZ from a Gram-positive bacterium, Bacillus subtilis, is reported. B. subtilis MinC inhibits FtsZ polymerization in a pH-dependent manner by preventing the formation of lateral associations between filaments. The inhibitory effect of MinC on FtsZ polymerization is cou...
متن کاملAssembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins.
FtsZ is the major cytoskeletal component of the bacterial cell division machinery. It forms a ring-shaped structure (the Z ring) that constricts as the bacterium divides. Previous in vivo experiments with green fluorescent protein-labeled FtsZ and fluorescence recovery after photobleaching have shown that the Escherichia coli Z ring is extremely dynamic, continually remodeling itself with a hal...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 181 4 شماره
صفحات -
تاریخ انتشار 1999